Resolution of heterogeneous fluorescence into component decay-associated excitation spectra. Application to subtilisins
نویسندگان
چکیده
منابع مشابه
Fluorescence technique for comparative studies of substrate-binding subsites in serine proteinases. Application to subtilisins.
A fluorescence technique for comparative studies of substrate-binding subsites in serine proteinases is described. It consists of: selective labelling of the corresponding subsites with a fluorescent group by using N alpha-dansyl(5-dimethylaminonaphthalene-1-sulphonyl)ated peptide chloromethanes containing different numbers of amino acid residues, and probing the immediate environment of the su...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1990
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(90)82521-2